Application | Comment | Organism |
---|---|---|
analysis | quick and sensitive enzyme detection assay based on the diphosphatase-coupled colorimetric method with malachite green, and assay for glucosamine-1-phosphate N-acetyltransferase activity of the bifunctional enzyme, both applicable in microtitter plates | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
UTP | pH 7.5, 37°C | Mycobacterium tuberculosis | |
0.044 | - |
N-acetyl-D-glucosamine 1-phosphate | pH 7.5, 37°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely required, optimal concentration 20 mM | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WMN3 | bifunctional UDP-N-acetylglucosamine diphosphorylase, EC 2.7.7.23, and glucosamine-1-phosphate N-acetyltransferase, EC 2.3.1.157 | - |
Mycobacterium tuberculosis H37Rv | P9WMN3 | bifunctional UDP-N-acetylglucosamine diphosphorylase, EC 2.7.7.23, and glucosamine-1-phosphate N-acetyltransferase, EC 2.3.1.157 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-glucosamine 1-phosphate + UTP | - |
Mycobacterium tuberculosis | UDP-N-acetyl-D-glucosamine + diphosphate | - |
? | |
N-acetyl-D-glucosamine 1-phosphate + UTP | - |
Mycobacterium tuberculosis H37Rv | UDP-N-acetyl-D-glucosamine + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlmU | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.04 | - |
UTP | pH 7.5, 37°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mycobacterium tuberculosis |